Parallel high-resolution confocal Raman SEM analysis of inorganic and organic bone matrix constituents
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A Kleindiek nano-positioning stage let Twente scan confocal Raman maps registered to SEM images — reading the mineral and protein chemistry of bone non-destructively.
The authors combine confocal Raman spectroscopy with scanning electron microscopy to map both the ultrastructure and the chemistry of bone matrix at high resolution, without destroying the sample. A Kleindiek nanometre scanning stage, driven under LabVIEW, rasters the specimen to build Raman images at chosen frequencies, which are then correlated with the SEM view of the very same region.